Czech Republic
Formed: 1901 Manager: Michal Bilek [CZE]
| Number | Name | Nat | Pos | Height | Weight | Date of Birth | Birth Place | Current Club |
| 1 | Petr Čech | CZE | G | 1.96 | 90 | 20-05-82 | Plzeň | Chelsea |
| 2 | Theodor Gebre Selassie | CZE | D | 1.82 | 70 | 24-12-86 | Třebíč | Slovan Liberec |
| 3 | Michal Kadlec | CZE | D | 1.83 | 75 | 13-12-84 | Vyškov | Bayer Leverkusen |
| 4 | Marek Suchý | CZE | D | 1.82 | 76 | 29-03-88 | Prague | Spartak Moscow |
| 5 | Roman Hubník | CZE | D | 1.91 | 84 | 06-06-84 | Vsetín | Hertha Berlin |
| 6 | Tomáš Sivok | CZE | D | 1.85 | 71 | 15-09-83 | Pelhřimov | Beşiktaş |
| 7 | Tomáš Necid | CZE | F | 1.90 | 81 | 13-08-89 | Prague | CSKA Moscow |
| 8 | David Limberský | CZE | D | 1.78 | 74 | 06-10-83 | Plzeň | Viktoria Plzeň |
| 9 | Jan Rezek | CZE | F | 1.77 | 74 | 05-05-82 | Most | Anorthosis Famagusta (On Loan) |
| 10 | Tomáš Rosický | CZE | M | 1.78 | 67 | 04-10-80 | Prague | Arsenal |
| 11 | Milan Petržela | CZE | M | 1.71 | 63 | 19-06-83 | Prague | Viktoria Plzeň |
| 12 | František Rajtoral | CZE | D | 1.77 | 75 | 12-03-86 | Příbram | Viktoria Plzeň |
| 13 | Jaroslav Plašil | CZE | M | 1.83 | 72 | 05-01-82 | Opočno | Bordeaux |
| 14 | Václav Pilař | CZE | M | 1.69 | 69 | 13-10-88 | Chlumec nad Cidlinou | Viktoria Plzeň (On Loan) |
| 15 | Milan Baroš | CZE | F | 1.84 | 78 | 28-10-81 | Valašské Meziříčí | Galatasaray |
| 16 | Jan Laštuvka | CZE | G | 1.91 | 87 | 07-07-82 | Havirov | Dnipro Dnipropetrovsk |
| 17 | Tomáš Hübschman | CZE | D | 1.80 | 74 | 04-09-81 | Prague | Shakhtar Donetsk |
| 18 | Daniel Kolář | CZE | M | 1.79 | 76 | 27-10-85 | Prague | Viktoria Plzeň |
| 19 | Petr Jiráček | CZE | M | 1.80 | 75 | 02-03-86 | Tuchořice | Wolfsburg |
| 20 | Tomáš Pekhart | CZE | F | 1.95 | 91 | 26-05-89 | Sušici | Nürnberg |
| 21 | David Lafata | CZE | F | 1.80 | 73 | 18-09-81 | České Budějovice | Jablonec |
| 22 | Vladimír Darida | CZE | M | 1.70 | 60 | 08-08-90 | Plzeň | Viktoria Plzeň |
| 23 | Jaroslav Drobný | CZE | G | 1.92 | 88 | 18-10-79 | Počátky | Hamburg |